Pyrex Journal of Research in Environmental Studies (PJRES)

February 2015 Vol. 2(2), pp. 008-012

Copyright 2015 Pyrex Journals

Full Length Research Paper

Assessment of Pesticide Toxicity Using The Freshwater Amoeba Rosculus ithacus in vitro

Hikal et al

*1Department of Biology, Faculty of Science, Tabuk University, P.O. Box 741, Tabuk 71491, Saudi Arabia 2Parasitology Laboratory, Water Pollution Research Department, Environmental Division, National Research Center, Dokki, 12622 Giza, Egypt
3Department of Zoology and Entomology, Faculty of Science, Helwan University, Helwan 11795, Egypt

Corresponding Author E-mail:

Accepted 16th January, 2015


Free-living amoebae are widely distributed in the aquatic environment with increasing importance in hygiene, medical and ecological relationships to man. Only few data are available concerning the behavior of this group of protozoa toward the accidental chemical changes in the aquatic environment. The inhibitory of 8 pesticides (methomyl, dimethoate, malathion, dicuran, cypermethrin, carbendazim, fenitrothion and butachlor) was estimated using the isolated and purified freshwater amoebae Rosculus ithacus. Toxicity experiments were carried out using short-term static relative sensitivity toxicity tests. Test organisms (Rosculus ithacus) were separately exposed to different concentrations (0.001, 0.004, 0.007, 0.01, 0.1 and 1 mg/l) from each of the selected 8 pesticides for 1, 10, 24, 48, 72 and 96 h. The mean inhibitory (IC50) concentrations of tested pesticides for Rosculus ithacus ranged from 0.0020 to 0.0064 mg/l. Concerning the inhibitory effect, for Rosculus ithacus butachlor was highly significantly more than malathion > carbendazim > methomyl > dimethoate > cypermethrin > dicuran > fenitrothion.

Keywords: Toxicity, pesticides, freshwater amoebae, Rosculus ithacus.

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